Fourteen people were injured when two passenger trains collided on track near Český Krumlov. Emergency helicopters attended the scene.
Around 50 passengers were on board the trains, according to police.
Damage to the trains is estimated at around 6.0 million crowns.
The around 80-kilometre stretch of line between České Budějovice and Černy Kříž is expected to remain closed into the mid-afternoon.
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